Retinal and olfactory bulb precursor cells show distinct responses to FGF-2 and laminin

Authors

  • Gaizka Otaegi,

    1. Group of Growth Factors in Vertebrate Development, Cellular and Molecular Physiopathology Department, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), C/Ramiro de Maeztu 9, E-28040 Madrid, Spain
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  • Flora De Pablo,

    1. Group of Growth Factors in Vertebrate Development, Cellular and Molecular Physiopathology Department, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), C/Ramiro de Maeztu 9, E-28040 Madrid, Spain
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  • Carlos Vicario-Abejón,

    1. Group of Growth Factors in Vertebrate Development, Cellular and Molecular Physiopathology Department, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), C/Ramiro de Maeztu 9, E-28040 Madrid, Spain
    2. Instituto Cajal, Consejo Superior de Investigaciones Científicas (CSIC), Av. Doctor Arce 37, E-28006 Madrid, Spain
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  • Enrique J. De, la Rosa

    Corresponding author
    1. Group of Growth Factors in Vertebrate Development, Cellular and Molecular Physiopathology Department, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), C/Ramiro de Maeztu 9, E-28040 Madrid, Spain
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Corresponding author. Tel.: +34 91 837 3112x4375; fax: +34 91 536 0432. ejdelarosa@cib.csic.es

Abstract

We analyzed whether the embryonic (E12.5–E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated proliferation and the formation of aggregates composed, after two days, of 95.2% nestin-positive cells. However, cells in these aggregates could only be passaged poorly, lost nestin expression and proliferative capacity, and differentiated into neurons. Under the same conditions, olfactory bulb precursor cells divided efficiently and could be expanded. These data suggest that, in addition to FGF-2 and laminin, embryonic retinal neuroepithelial cells need additional extrinsic and/or intrinsic regulators to maintain cell proliferation and self-renewal.

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