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Keywords:

  • Kidney mitochondria;
  • Permeability transition;
  • Calcium;
  • Copper;
  • Carboxyatractyloside

Abstract

In this work we examined the effect of low concentrations of Cu2+ on the opening of the mitochondrial non-specific pore. The purpose was addressed to further contribute to the knowledge of the mechanisms that regulate the open/closed cycles of the permeability transition pore. Membrane leakage was established by measuring matrix Ca2+ efflux and mitochondrial swelling. The experimental results indicate that Cu2+ at very low concentrations promoted the release of accumulated Ca2+, as well as mitochondrial swelling, provided 1,10-phenanthroline has been added. Carboxyatractyloside and Cu2+ exhibited additive effects on these parameters. After Cu2+ titration of membrane thiols, it might be assumed that the blockage of 5.9 nmol of SH/mg protein suffices to open the non-specific pore. Taking into account the reinforcing effect of carboxyatractyloside, the increasing ADP concentrations, and that N-ethylmaleimide inhibited the Cu2+-induced Ca2+ efflux, it is proposed that the target site for Cu2+ is located in the ADP/ATP carrier.