Influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV) in insect Spodoptera litura cells

Authors

  • Lijun Liu,

    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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  • Jianxin Peng,

    Corresponding author
    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
    2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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  • Kaiyu Liu,

    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
    2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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  • Hong Yang,

    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
    2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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  • Yi Li,

    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
    2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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  • Huazhu Hong

    1. Institute of Entomology, Ministry of Education, Central China Normal University, Wuhan 430079, China
    2. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China
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Corresponding author. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China. Tel.: +86 027 6786 2431. Jianxinpeng@21cn.com

Abstract

We investigated the influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL-1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of AfMNPV-infected SL-1cells. Western blot analysis demonstrated that AfMNPV-induced apoptosis in insect SL-1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC-DEVD-AFC as substrate, the activity of caspase-3 in AfMNPV-induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase-3 in apoptotic cells decreased in the presence of cyclosporin A (30 μM), indicating that activation of caspase-3 in SfaMNPV-induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (ΔΨm) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV-induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis.

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