In vivo dedifferentiation of human epidermal cells

Authors

  • Haihong Li,

    1. Mental Health Center, ShanTou University Medical College, GuangDong Province 515063, PR China
    2. Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Trauma Center of Postgraduate Medical College, 51 Fu Cheng Road, Beijing 100037, PR China
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  • Xiaobing Fu,

    Corresponding author
    1. Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Trauma Center of Postgraduate Medical College, 51 Fu Cheng Road, Beijing 100037, PR China
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  • Lei Zhang,

    1. Mental Health Center, ShanTou University Medical College, GuangDong Province 515063, PR China
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  • Tongzhu Sun,

    1. Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Trauma Center of Postgraduate Medical College, 51 Fu Cheng Road, Beijing 100037, PR China
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  • Jun Wang

    1. Wound Healing and Cell Biology Laboratory, Burns Institute, The First Affiliated Hospital (304th Hospital), General Hospital of PLA, Trauma Center of Postgraduate Medical College, 51 Fu Cheng Road, Beijing 100037, PR China
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Corresponding author. Tel.: +86 10 6686 7396; fax: +86 10 6848 0755. fuxb@cgw.net.cn, fuxiaobing@vip.sina.com

Abstract

Consistent with our previous study, we herein offer further evidence to demonstrate the dedifferentiation of differentiating epidermal cells into stem cells or stem cells -like in vivo. The epidermal sheets eliminated of basal cells were labeled with 6-diamidino-2-phenylindole (DAPI), and then were transplanted onto the full-thickness skin wounds nude mice. Immunohistochemical examination of the survival sheets showed that some cells were positive for both DAPI and either cytokeratins (CK19, CK14) or β1 integrin in spinous and granular layers at day 7 after transplantation. Furthermore, there was a significant increase in the percentages of both α6briCDdim and α6briCD71bri populations in survival epidermal sheet grafts 7 d after transplantation compared with those before xenotransplantation (P < 0.05), as determined by flow cytometry. The results collectively indicated that some of the differentiated cells in engrafted epidermal sheets dedifferentiated into stem cells or stem cells-like in vivo, which offer us new evidence and insights into the dedifferentiation.

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