Biochemical study of idebenone effect on mitochondrial metabolism of yeast

Authors

  • Sebastián P. Chapela,

    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
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  • Hilda I. Burgos,

    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
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  • Ana I. Salazar,

    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
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  • Inés Nievas,

    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
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  • Norberto Kriguer,

    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
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  • Carlos A. Stella

    Corresponding author
    1. Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 5, Argentina
      Corresponding author. Fax: +54 011 4508 3672x31. cstella@fmed.uba.ar
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Corresponding author. Fax: +54 011 4508 3672x31. cstella@fmed.uba.ar

Abstract

The aim of this work was to study the effect of the drug idebenone on the growth of a strain of Saccharomyces cerevisiae yeast and its respiratory-deficient mutant (rho0). We took this yeast as a model system of the interaction of the drug with mammalian cells. The effect of idebenone was evaluated in rich and minimal media. In the S288c strain, idebenone exerted a growth inhibitory effect in concentrations higher than 50 μM in media containing a carbon source consumed at mitochondrial level. In conditions of low oxygen supply, idebenone allows yeast to keep a cellular yielding comparable with conditions of normal oxygen supply. Also, the presence of idebenone in the growth media increased by 50% the fluorescence signal of rhodamine 123, indicating a higher mitochondrial membrane potential. The results could explain the effect of idebenone in the treatment of diseases in which oxygen deficiency alters the energetic metabolism of the cell.

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