Evaluation of chemokine and cytokine profiles in osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology

Authors

  • Letizia Penolazzi,

    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Elisabetta Lambertini,

    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Elisa Tavanti,

    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Elena Torreggiani,

    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Fortunato Vesce,

    1. Department of Biomedical Sciences and Advanced Therapies, Section of Obstetric and Gynaecological Clinic, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Roberto Gambari,

    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
    2. Biotechnology Center, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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  • Roberta Piva

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, Molecular Biology Section, University of Ferrara, Via Fossato di Mortara, 74, Ferrara, Italy
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Corresponding author. Tel.: +39 532 424 405. piv@unife.it

Abstract

We have used cytokine protein array to analyze the secretion of cytokines from an osteoblastic clone derived from human umbilical cord blood mesenchymal stem cells (MSCs) cultured in an osteogenic differentiation medium. The analysis demonstrated the unexpected ability of osteoblast committed cells and their early progenitors to produce significant amounts of a range of soluble immune mediators without in vitro exposure to clinically relevant bacterial pathogens. The cells were expanded and their osteogenic potential analyzed over 45 days of culture was revealed by the expression of osteoblast-specific markers (alkaline phosphatase and Runx2), and by matrix mineralization. Over this culture period, the cells secreted particularly high levels of IL-8, MCP-1 and VEGF, but did not express IL-2, IL-7, IL-17, eotaxin, G-CSF and IFN-γ. These findings should encourage the use of human umbilical cord blood as a potential stem cells source for bone regeneration.

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