Cloning and expression of the tubulin genes in barley

Authors

  • Volodymyr Radchuk,

    Corresponding author
    1. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany
    2. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kiev 03143, Ukraine
      Corresponding author. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany. Tel.: +49 (0) 39 485 536; fax: +49 (0) 39 485 500. radchukv@ipk-gatersleben.de
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  • Nese Sreenivasulu,

    1. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany
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  • Yaroslav Blume,

    1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kiev 03143, Ukraine
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  • Winfriede Weschke

    1. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany
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Corresponding author. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), D-06466 Gatersleben, Germany. Tel.: +49 (0) 39 485 536; fax: +49 (0) 39 485 500. radchukv@ipk-gatersleben.de

Abstract

Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for α-, β-, and γ-tubulins were selected. Five genes for α-tubulin, eight newly identified β-tubulin sequences and one γ-tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific.

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