Polycystin-1 induced apoptosis and cell cycle arrest in G0/G1 phase in cancer cells

Authors

  • Rong Zheng,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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    • These authors contributed equally to this work.

  • Zheng Zhang,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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    • These authors contributed equally to this work.

  • Xiaoyan Lv,

    1. Department of Dermatology, West China Hospital, Sichuan University, Chengdu, PR China
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  • JunMing Fan,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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  • Ye Chen,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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  • Yidong Wang,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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  • Ruizhi Tan,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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  • Yuhang Liu,

    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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  • Qin Zhou

    Corresponding author
    1. Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China
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Corresponding author. Tel./fax: +86 28 8512 5449. pkdzhou@126.com

Abstract

Studies have shown that polycystin-1, encoded by PKD1, the major ADPKD, may have a central role in regulating both apoptosis and proliferation, which could prevent the malignant transformation of affected cells. However, as a putative tumor suppressor, direct studies on the possibility that polycystin-1 may play a role in cancer cells' biological properties have not yet been reported. We have demonstrated that the apoptosis of cancer cells was induced by overexpression of polycystin-1. After transfection with polycystin-1, three cancer cell lines, HepG2, A549, and SW480, showed significantly increased apoptosis compared with the respective control groups. This was accompanied by cell cycle arrest at G0/G1 phase, whereas cell proliferation was not significantly affected. Overexpression of polycystin-1 induces apoptosis in cancer cells, at least partially, through Wnt and a caspase-dependent pathway.

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