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Flow cytometric cell cycle analysis of cultured brown bear fibroblast cells
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2008 International Federation for Cell Biology
Cell Biology International
Volume 32, Issue 7, pages 855–859, July 2008
How to Cite
Caamaño, J.N., Rodriguez, A., Salas, A., Muñoz, M., Diez, C., Prather, R.S. and Gómez, E. (2008), Flow cytometric cell cycle analysis of cultured brown bear fibroblast cells. Cell Biology International, 32: 855–859. doi: 10.1016/j.cellbi.2008.02.005
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 9 October 2007; revised 13 November 2007; accepted 25 February 2008
- Brown bear;
- Cell cycle;
- Flow cytometry;
The aim of this study was to assess by flow cytometry the cell cycle of brown bear fibroblast cells cultured under different growth conditions. Skin biopsies were taken in Cantabria (Spain) from a live, anaesthetized brown bear. DNA analysis was performed by flow cytometry following cell DNA staining with propidium iodide. Serum starvation increased (P < 0.01) the percentage of G0/G1 phase cells (92.7 ± 0.86) as compared to cycling cells (39.7 ± 0.86) or cells cultured to confluency (87.3 ± 0.86). DMSO included for 48 h in the culture significantly increased (P < 0.01) the percentage of G0/G1 phase of the cell cycle at all concentrations used and decreased percentages of S phase in a dose-dependent fashion. Roscovitine increased the G0/G1 phase of the cell cycle (P < 0.01) at 15 μM concentration. Interestingly, the G2/M stage significantly increased at 30 and 50 μM compared to the control and 15 μM (P < 0.02). The cell cycle of brown bear adult fibroblast cells can be successfully synchronized under a variety of culture conditions.