Participation of Rho, ROCK-2, and GAP activities during actin microfilament rearrangements in Entamoeba histolytica induced by fibronectin signaling

Authors

  • Amelia Ríos,

    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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    • A.R. and V.I.H.R. contributed equally to this work.

    • Present address: Cinvestav-IPN, Unidad Monterrey, Monterrey N.L., Mexico.

  • Verónica Ivonne Hernández-Ramírez,

    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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    • A.R. and V.I.H.R. contributed equally to this work.

  • Mayra Moguel,

    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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    • Present address: Laboratorio de Genética Forense, Procuraduría General de Justicia del Estado, Carretera Chihuahua-Aldama Km 3.5 s/n, Chihuahua, Chih., Mexico.

  • Alma Iris Zárate Bahena,

    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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    • Present address: Universidad Autónoma de Guerrero, Facultad de Medicina, Av. Solidaridad S/N, Col. Insurgentes, Acapulco, Gro., Mexico.

  • José Luis Rosales-Encina,

    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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  • Miguel Angel Vargas,

    1. Departamento de Biomedicina Molecular, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
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  • Patricia Talamás-Rohana

    Corresponding author
    1. Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico
      Corresponding author. Tel.: +52 55 5747 3351; fax: +52 55 5747 3377. ptr@cinvestav.mx
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Corresponding author. Tel.: +52 55 5747 3351; fax: +52 55 5747 3377. ptr@cinvestav.mx

Abstract

In Entamoeba histolytica little is known about the microfilament rearrangements formed by actin and ABPs. Fibronectin regulates many aspects of cell behavior involving the actin cytoskeleton and members of the Rho family of small GTPases. Using trophozoites interacted with fibronectin and glass, we present evidence related with the formation and regulation of different microfilament rearrangements and their cellular distribution, the effect of actin affecting drugs on these arrangements, and on trophozoites adhesion; we also demonstrate that actin isoforms are induced after adhesion, and also the selective participation of specific actin binding proteins such as ABP-120 and phospho-paxillin, regarding their location in the different actin structures. In addition, we show results that confirm the participation of EhRho, ROCK-2, and GAP activities. We propose that fibronectin induced signaling in E. histolytica trophozoites have important consequences in the actin cytoskeleton that may affect its behavior during the invasive process in the host.

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