Calcium near the release site is essential for basal ACh release in Xenopus

Authors

  • Ruxin Li,

    1. Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
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  • Qi Lei,

    1. Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
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  • Ge Song,

    1. Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
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  • Xiangping He,

    1. Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
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  • Zuoping Xie

    Corresponding author
    1. Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
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Corresponding author. Tel.: +86 10 6278 8677; fax: +86 10 6277 2271. zuopingx@tsinghua.edu.cn

Abstract

Extracellular calcium is essential for neurotransmitter release, but the detailed mechanism by which Ca2+ regulates basal synaptic release has not yet been fully explored. In this study, calcium imaging and the whole-cell patch-clamp technique were used to investigate the role of Ca2+ in basal acetylcholine (ACh) release in the Xenopus neuromuscular junction and in isolated myocytes exogenously loaded with ACh. Carried out in normal and Ca2+-free extracellular solution, the results indicate that Ca2+ near the release site is essential for basal neurotransmitter release.

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