Both authors contributed equally to this study.
Combination of bone tissue engineering and BMP-2 gene transfection promotes bone healing in osteoporotic rats
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2008 International Federation for Cell Biology
Cell Biology International
Volume 32, Issue 9, pages 1150–1157, September 2008
How to Cite
Tang, Y., Tang, W., Lin, Y., Long, J., Wang, H., Liu, L. and Tian, W. (2008), Combination of bone tissue engineering and BMP-2 gene transfection promotes bone healing in osteoporotic rats. Cell Biology International, 32: 1150–1157. doi: 10.1016/j.cellbi.2008.06.005
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 20 November 2007; revised 10 April 2008; accepted 22 June 2008
- Bone regeneration;
- Bone morphogenetic protein 2;
- Tissue engineering;
- Gene therapy
Objective: The aim of this study was to develop a feasible approach to promote bone healing in osteoporotic rats using autogenous bone tissue-engineering and gene transfection of human bone morphogenetic protein 2 (hBMP-2).
Methods: Bone marrow stromal cells (BMSCs) from the left tibia of osteoporotic rats were transfected with the hBMP-2 gene in vitro which was confirmed by immunohistochemistry, in situ hybridization and Western blotting. Autogenous transfected or untransfected BMSCs were seeded on macroporous coral hydroxyapatite (CHA) scaffolds. Each cell-scaffold construct was implanted into a defect site which was created in the ramus of the mandible of osteoporotic rats. Four or eight weeks after implantation in situ hybridization was performed in BMSCs transfected with hBMP-2, X-ray examinations, histological and histomorphological analyses were used to evaluate the effect of tissue-engineered bone on osseous defect repair.
Results: Newly formed bone was observed at the margin of the defect 4 weeks after implantation with BMSCs transfected with BMP-2. Mature bone was observed 8 weeks after treatment. In the control group there was considerably less new bone and some adipose tissue was observed at the defect margins 8 weeks after implantation.
Conclusions: Autogenous cells transfected with hBMP-2 promote bone formation in osteoporotic rats. BMSC-mediated BMP-2 gene therapy used in conjunction with bone tissue engineering may be used to successfully treat bone defects in osteoporotic rats. This method provides a powerful tool for bone regeneration and other tissue engineering.