Puerarin protects PC12 cells against β-amyloid-induced cell injury

Authors

  • Hai-Ying Zhang,

    1. Department of Human Anatomy and Histology & Embryology, Medical School of Xi'an Jiaotong University, Xi'an, PR China
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    • These authors contributed equally to this work.

  • Yi-Heng Liu,

    1. Department of Orthopedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China
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    • These authors contributed equally to this work.

  • Hong-Quan Wang,

    1. Department of Human Anatomy and Histology & Embryology, Medical School of Xi'an Jiaotong University, Xi'an, PR China
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  • Jie-Hua Xu,

    1. Department of Human Anatomy and Histology & Embryology, Medical School of Xi'an Jiaotong University, Xi'an, PR China
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  • Hai-Tao Hu

    Corresponding author
    1. Department of Human Anatomy and Histology & Embryology, Medical School of Xi'an Jiaotong University, Xi'an, PR China
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Corresponding author. Department of Anatomy and Histology & Embryology, Medicine School of Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, Shaanxi 710061, PR China. Tel.: +86 29 8265 5426; fax: +86 29 865 95130. hyzhang_xjtu@yahoo.cn

Abstract

β-Amyloid protein (Aβ), a major protein component of brain senile plaques in Alzheimer's disease, is known to be directly responsible for the production of reactive oxygen species (ROS) and induction of apoptosis. In this study, the protective effect of puerarin, an isoflavone purified from the radix of the Chinese herb Pueraria lobata, on Aβ-induced rat pheochromocytoma (PC12) cultures was investigated. Although exposure of PC12 cells to 50 μM Aβ25–35 caused significant viability loss and apoptotic rate increase, pretreatment of the cells with puerarin for 24 h reduced the viability loss and apoptotic rate. Puerarin (1 μM) significantly inhibited Aβ25–35-induced apoptosis of PC12 cells. Preincubation of the cell with puerarin also restored the ROS and mitochondrial membrane potential levels that had been altered as a result of Aβ25–35 treatment. Puerarin was also found to increase the Bcl-2/Bax ratio and reduce caspase-3 activation. These results suggest that puerarin could attenuate Aβ25–35-induced PC12 cell injure and apoptosis and could also promote the survival of PC12 cells. Therefore, puerarin may act as an intracellular ROS scavenger, and its antioxidant properties may protect against Aβ25–35-induced cell injury.

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