Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells

Authors

  • Maryam Jazayeri,

    1. Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
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  • Abdolamir Allameh,

    Corresponding author
    1. Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
      Corresponding author. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-331, Tehran, Iran. Tel: +98 21 88013030; fax: +98 21 88006544. allameha@modares.ac.ir
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  • Masoud Soleimani,

    1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
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  • Seyed Hamid Jazayeri,

    1. Department of Pathology, National Iranian Oil Corporation (NIOC) Central Hospital, Tehran, Iran
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  • Abbas Piryaei,

    1. Cell and Molecular Biology Research Center, Department of Anatomy, Faculty of Medical Sciences, Shaheed Beheshti University, Tehran, Iran
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  • Somaieh Kazemnejad

    1. Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
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Corresponding author. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-331, Tehran, Iran. Tel: +98 21 88013030; fax: +98 21 88006544. allameha@modares.ac.ir

Abstract

In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development.

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