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Interactions of Taxol-producing endophytic fungus with its host (Taxus spp.) during Taxol accumulation

Authors

  • Yong-Cheng Li,

    1. Key Laboratory of Industrial Biotechnology of the Ministry Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
    2. School of Food, Hainan University, Haikou 570228, China
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  • Wen-Yi Tao

    Corresponding author
    1. Key Laboratory of Industrial Biotechnology of the Ministry Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
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Fax: +86 0510 8580 6493. E-mail addresses: wytao1946@163.com

Abstract

Endophytic fungi (Fusarium mairei) culture broth (EFCB) was added to cell suspension cultures of Taxus cuspidata. After 5 days, cultures of T. cuspidata given 4 ml of EFCB produced a maximal yield of 6.11 mg/l paclitaxel, with a release ratio of 75%, 2- and 6.8-fold, respectively, greater than the controls. The active element in EFCB is an exopolysaccharide of ∼79 kD. Endophytic fungi produced 0.19 mg/l of paclitaxel in its producing medium. However, when the supernatant of Taxus cell suspension cultures from day 20 was added to the paclitaxel-producing medium, the biomass of fungi decreased by 24% and the yield of paclitaxel by 45%. In a co-culture system of plant and fungus, the yield of paclitaxel (12.8 mg/l) was >2-fold higher than that in the EFCB-treatment system.

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