Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs)
Article first published online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 1, pages 100–105, January 2009
How to Cite
Passeri, S., Nocchi, F., Lamanna, R., Lapi, S., Miragliotta, V., Giannessi, E., Abramo, F., Stornelli, M. R., Matarazzo, M., Plenteda, D., Urciuoli, P., Scatena, F. and Coli, A. (2009), Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs). Cell Biology International, 33: 100–105. doi: 10.1016/j.cellbi.2008.10.012
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 11 June 2008; revised 18 September 2008; accepted 13 October 2008
- Umbilical cord-derived matrix cells;
- Umbilical cord;
- Stem cells;
- Epidermal growth factor
Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy.
EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in α-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to α-MEM.
α-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of α-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol.
Both α-MEM and MSCBM can be used to cultivate EUCMCs. α-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since α-MEM is more cost-effective than MSCBM.