These authors have made equal contributions to this article as first authors.
Effects of substance P on osteoblastic differentiation and heme oxygenase-1 in human periodontal ligament cells
Article first published online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 3, pages 424–428, March 2009
How to Cite
Kook, Y.-A., Lee, S.-K., Son, D.-H., Kim, Y., Kang, K.-H., Cho, J.-H., Kim, S.-C., Kim, Y.-S., Lee, H.-J., Lee, S.-K. and Kim, E.-C. (2009), Effects of substance P on osteoblastic differentiation and heme oxygenase-1 in human periodontal ligament cells. Cell Biology International, 33: 424–428. doi: 10.1016/j.cellbi.2008.12.007
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 25 July 2008; revised 28 August 2008; accepted 5 December 2008
- Substance P;
- PDL cells;
- Osteogenic differentiation
Although substance P (SP) is associated with osteoclast differentiation and bone resorption, little is known about the osteogenic differentiation-inducing effects of SP in periodontal ligament (PDL) cells. This study investigated whether PDL cells could differentiate into osteoblastic-like cells by SP. The expression of osteoblastic differentiation markers such as osteopontin (OPN), osteonectin (ON), osteocalcin (OCN) and bone sialoprotein (BSP) were evaulated by Western blotting. Additionally, SP-mediated heme oxygenase-1 (HO-1) pathways were further clarified.
SP increased HO-1 and osteogenic differentiation in concentration- and time-dependent manners, as determined by OPN, ON, OCN and BSP expression. Furthermore, treatment with inhibitors of p38, ERK MAPK, and NF-κB abolished SP-induced osteogenic differentiation and HO-1 expression. SP-induced translocation of Nrf-2 was also observed. The combined results suggest that SP activates the stress-response enzymes HO-1 and Nrf-2, subsequently leading to upregulation of osteogenic differentiation in human PDL cells.