Linker histone-like proteins in Muscovy duck (Cairina moschata L) erythrocyte chromatin
Version of Record online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 3, pages 344–351, March 2009
How to Cite
Kowalski, A., Pałyga, J. and Górnicka-Michalska, E. (2009), Linker histone-like proteins in Muscovy duck (Cairina moschata L) erythrocyte chromatin. Cell Biology International, 33: 344–351. doi: 10.1016/j.cellbi.2009.01.002
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Received 8 May 2008; revised 1 October 2008; accepted 9 January 2009
- Linker histones;
- Liquid chromatography–linear quadrupole ion trap–Fourier transform mass spectrometry;
- Muscovy duck;
- Polyacrylamide gel electrophoresis;
Linker Histone-Like proteins (LHL1 and LHL2) were identified within a linker histone complement of Muscovy duck erythrocyte chromatin. Polyacrylamide gel electrophoretic patterns of N-bromosuccinimide-cleaved LHL products as well as liquid chromatography-electrospray-ion trap mass spectrometry analyses of trypsin-digested LHL peptides revealed structural similarity of LHL1 to histone H5 and between LHL2 and histone H1 subtypes.
Since the LHL proteins were stable in the presence of 2-mercaptoethanol and dithiothreitol that reduce disulfide bonds, it appeared unlikely that this doublet was a thiol-derived product of linker histones. A loss of LHL1, with a concomitant maintenance of LHL2 after treatment with dilute alkali, seems to suggest that they might represent disparate protein conjugates resulting from linker histone modifications through ester linkages.