Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Authors

  • Jun-Jie Wang,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Xiu-Qing Fu,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Yu-Guang Guo,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Lin Yuan,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Qian-Qian Gao,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Hua-Li Yu,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Heng-Liang Shi,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Xing-Zhi Wang,

    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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  • Wen-Cheng Xiong,

    1. Program of Developmental Neurobiology, IMMAG and Department of Neurology, Medical College of Georgia, Augusta, GA 30912, USA
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  • Xiao-Juan Zhu

    Corresponding author
    1. Institute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
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Tel.: +86 431 85099769; fax: +86 431 85099822. E-mail addresses: xiaojuan5183@yahoo.com

Abstract

Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

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