These authors contributed equally to the work.
Overexpression of E2F-1 inhibits progression of gastric cancer in vitro
Article first published online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 6, pages 640–649, June 2009
How to Cite
Xie, Y., Wang, C., Li, L., Ma, Y., Yin, Y. and Xiao, Q. (2009), Overexpression of E2F-1 inhibits progression of gastric cancer in vitro. Cell Biology International, 33: 640–649. doi: 10.1016/j.cellbi.2009.02.015
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 16 December 2008; accepted 25 February 2009
- Gastric cancer;
- MGC-803 cells;
- Tumor progression
E2F-1 plays a critical role in cell cycle regulation and other biological processes in cells. E2F-1 mediates apoptosis and suppresses tumorigenesis in many tissue types, but there are few data available on E2F-1 expression and its relationship to tumor kinetics in gastric cancer. To gain better insight into the involvement of E2F-1 in the biological characteristics of gastric tumors, we investigated the effect of E2F-1 overexpression on the progression of gastric carcinoma cells. A gastric cancer cell line stably overexpressing E2F-1 (MGC-803/E2F-1) was established. The influence of E2F-1 overexpression on in vitro cell growth was assessed by measuring cell survival, colony formation, and cell cycle progression. The results clearly show that overexpression of E2F-1 significantly inhibits cell growth and proliferation, blocking entry into the S-phase of the cell cycle. MGC-803/E2F-1 cells also had a higher apoptotic rate than control cells. In addition, E2F-1 reduced the motility and invasion of gastric cancer cells.