Both author contributed equally to this work.
Downregulation of CD2-associated protein impaired the physiological functions of podocytes
Article first published online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 6, pages 632–639, June 2009
How to Cite
Zhang, C., Jiang, H.-J., Chang, Y., Fang, Z., Sun, X.-F., Liu, J.-S., Deng, A.-G. and Zhu, Z.-H. (2009), Downregulation of CD2-associated protein impaired the physiological functions of podocytes. Cell Biology International, 33: 632–639. doi: 10.1016/j.cellbi.2009.02.017
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 6 June 2008; revised 12 October 2008; accepted 25 February 2009
Emerging evidences show that CD2-associated protein (CD2AP) is involved in podocyte injury and the pathogenesis of proteinuria. However, the exact molecular mechanism by which CD2AP exerts its biological function is elusive. We knocked down CD2AP gene by target siRNA in conditionally immortalized mouse podocytes, which showed lowered cell adhesion and spreading ability (P < 0.05). At the same time, cell cycle was arrested in G2/M phase (P < 0.05), and pathologic nuclear division could easily be seen in CD2AP siRNA-transfected podocytes. The proliferation of podocytes were also inhibited significantly by CD2AP siRNA transfection (P < 0.05). Further study revealed disordered distributions of F-actin, as well as lowered nephrin expression and phosphorylation in podocytes. These data suggest that CD2AP may play a crucial role in maintaining the normal function of podocytes and lowered CD2AP causes podocyte injury by disrupting the cytoskeleton and disturbing the nephrin-CD2AP signaling pathway.