Get access

IGF-1 and G-CSF complement each other in BMSC migration towards infarcted myocardium in a novel in vitro model

Authors

  • Mohsin Khan,

    1. National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
    Search for more papers by this author
  • Sobia Manzoor,

    1. National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
    Search for more papers by this author
  • Sadia Mohsin,

    1. National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
    Search for more papers by this author
  • Shaheen N. Khan,

    1. National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
    Search for more papers by this author
    • Shaheen N. Khan and Fridoon J. Ahmad contributed equally to this project.

  • Fridoon Jawad Ahmad

    Corresponding author
    1. National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
      Tel.: +92 300 846 3801; fax: +92 42 572 2694. E-mail addresses: drfridoon@yahoo.com
    Search for more papers by this author
    • Shaheen N. Khan and Fridoon J. Ahmad contributed equally to this project.


Tel.: +92 300 846 3801; fax: +92 42 572 2694. E-mail addresses: drfridoon@yahoo.com

Abstract

Stem cell capability enhanced with cytokine administration is a promising treatment for myocardial infarction. Bone marrow stem cells (BMSCs) were isolated from C57BL/6 mice (8–12 weeks old) expressing GFP and characterized with c-kit and CD34. Infarcted heart tissue fragments were placed into dishes with BMSCs and medium supplemented with G-CSF, SCF, IGF-1 or combinations thereof were given to the BMSC-infarcted myocardium in vitro model. The IGF-1–G-CSF group showed significantly higher migration (67.7% ± 2.6) of c-kit+ BMSCs towards the ischemic tissue and expressed MEF-2 (43.7% ± 1.7). Of the single treatment groups, the G-CSF group demonstrated significantly higher migration of c-kit+ BMSCs (60.5 ± 2.7) with MEF-2 expression (38.7 ± 1.4). IGF-1 complements G-CSF and was relatively more significant in its effects on BMSC migration and cardiac lineage commitment towards ischemic heart tissue.

Get access to the full text of this article

Ancillary