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Proteomics and transcriptome approaches to investigate the mechanism of human sex determination

Authors

  • Youichi Sato,

    Corresponding author
    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Toshikatsu Shinka,

    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Gang Chen,

    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Hong-Tao Yan,

    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Kozue Sakamoto,

    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Ashraf A. Ewis,

    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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  • Hiroyuki Aburatani,

    1. Genome Science Division, Center for Collaborative Research, University of Tokyo, Japan
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  • Yutaka Nakahori

    Corresponding author
    1. Department of Human Genetics and Public Health, Graduate School of Proteomics, Faculty of Medicine, University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima 770-8503, Japan
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Tel.: +81 88 633 7075; fax: +81 88 633 7453. E-mail addresses: sato@basic.med.tokushima-u.ac.jp

E-mail addresses: nakahori@basic.med.tokushima-u.ac.jp

Abstract

The SRY gene (sex-determining region on the Y chromosome) was isolated in 1990 and is known as the testis-determining factor on the Y chromosome. The SRY has been considered as a transcription factor since it contains an HMG box, which functions as a DNA-binding domain. However, a direct target for SRY remains to be identified. We have investigated the function of SRY through proteomics and transcriptome approaches, and by using two stable SRY-overexpressing cell lines (SRY1 and SRY2) in NT2/D1 cells derived from human testicular embryonal cell carcinoma. The results of 2-dimensional gel electrophoresis show that SRY overexpression causes a considerable downregulation of many chaperone proteins. SRY also upregulates laminin, which is important for Sertoli cell differentiation. Additionally, transcriptome analysis shows that SRY overexpression upregulates many zinc finger proteins and downregulates cellular growth factors with S or G2/M arrest of the cell cycle and inhibition of cellular proliferation.

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