BMP-2 treatment of C3H10T1/2 mesenchymal cells blocks MMP-9 activity during chondrocyte commitment

Authors

  • Young-Ae Choi,

    1. Department of Biological Sciences, College of Natural Sciences, Kyungbook National University, Daegu 702-701, South Korea
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  • Shin-Sung Kang,

    1. Department of Biological Sciences, College of Natural Sciences, Kyungbook National University, Daegu 702-701, South Korea
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  • Eun-Jung Jin

    Corresponding author
    1. Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Chunbuk, 570-749, South Korea
    2. Institute of Biotechnology, Wonkwang University, Iksan, Chunbuk, 570-749, South Korea
      Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Chunbuk, 570-749, South Korea. Fax: +82 63 857 8837. E-mail addresses: jineunjung@wku.ac.kr
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Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Chunbuk, 570-749, South Korea. Fax: +82 63 857 8837. E-mail addresses: jineunjung@wku.ac.kr

Abstract

Members of both the Wnt and bone morphogenetic protein (BMP) families of signaling molecules have been implicated in the regulation of cartilage development. We explored the underlying mechanism of BMP-2-induced chondrocyte commitment of C3H10T1/2 cells. Treating cells with exogenous BMP-2 was tied to chondrocyte commitment by inhibiting matrix metalloproteinase-9 activity (MMP-9: 92 kDa type IV collagenase/gelatinase B). Glycogen synthase kinase (GSK)-3β inhibition by its specific inhibitor blocked BMP-2-induced chondrocyte commitment by stimulating MMP-9 activity. These findings indicate that the downregulation of MMP-9 by BMP-2 is associated with chondrocyte commitment, and that the GSK-3β signaling pathway is involved in this process.

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