Clinical grade mesenchymal stem cells transdifferentiated under xenofree conditions alleviates motor deficiencies in a rat model of Parkinson's disease
Article first published online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 8, pages 830–838, August 2009
How to Cite
Shetty, P., Ravindran, G., Sarang, S., Thakur, A. M., Rao, H. S. and Viswanathan, C. (2009), Clinical grade mesenchymal stem cells transdifferentiated under xenofree conditions alleviates motor deficiencies in a rat model of Parkinson's disease. Cell Biology International, 33: 830–838. doi: 10.1016/j.cellbi.2009.05.002
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 14 August 2008; revised 13 January 2009; accepted 7 May 2009
- Bone marrow;
- Clinical grade mesenchymal stem cells (MSCs);
- Cord blood serum (CBS);
- Dopaminergic neurons;
- 6-Hydroxydopamine (6-OHDA);
- Parkinson's disease (PD)
Bone marrow derived mesenchymal stem cells (BMMSCs) is a valid, definitive candidate for repair of damaged tissues in degenerative disorders in general and neurological diseases in particular. We have standardized the processing conditions for proliferation of BMMSCs using xenofree medium and checked their in vitro and in vivo neurogenic potential.
Method: The proliferative potential of BMMSCs was analyzed using xenofree media and functionality checked by transplantation into Parkinson's disease (PD) animal models. In vitro neuronal differentiation was investigated by neuronal induction media supplemented with growth factors. Differentiated cells were characterized at cellular and molecular levels. In vitro functionality estimated by dopamine secretion.
Results: A pure population of BMMSCs showing an 8–10 fold expansion was obtained using xenofree media. On differentiation to neuronal lineage, they exhibited neuronal morphology. Detectable levels of dopamine (1.93 ng/ml) were secreted into the culture media of differentiated cells. There was a significant behavioural improvement in PD models 3 months post transplantation.
Conclusion: Our study demonstrates that BMMSCs can be transdifferentiated efficiently into functional dopaminergic neurons both in vitro and in vivo. This holds immense clinical potential as a replacement therapy for PD and other neurodegenerative diseases.