Resistance of a recombinant Escherichia coli to dehydration

Authors

  • Galina Khroustalyova,

    1. Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Kronvald Blvd., 4, LV-1586 Riga, Latvia
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  • Joseph Boudrant,

    1. Laboratory of Chemical Engineering Science, UPR CNRS 6811, Nancy-Université, INPL, Vandoeuvre-les-Nancy, France
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  • Alexander Rapoport

    Corresponding author
    1. Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Kronvald Blvd., 4, LV-1586 Riga, Latvia
      Tel.: +371 67034891; fax: +371 67034885. E-mail addresses: rapoport@mail.eunet.lv
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Tel.: +371 67034891; fax: +371 67034885. E-mail addresses: rapoport@mail.eunet.lv

Abstract

Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a ∼88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).

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