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Antheraea pernyi silk fibroin maintains the immunosupressive properties of human bone marrow mesenchymal stem cells

Authors

  • Xi-Ying Luan,

    Corresponding author
    1. Department of Immunology, Binzhou Medical college, Shandong Province, Yantai 264003, People's Republic of China
    2. Institute of Medical Biotechnology, Suzhou University; Jiangsu Province Key Laboratory of Stem Cell, Suzhou 215007, People's Republic of China
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  • Guan-Hua Huo,

    1. Department of Immunology, Binzhou Medical college, Shandong Province, Yantai 264003, People's Republic of China
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  • Ming-Zhong Li,

    1. School of Materials Engineering, Suzhou University, Suzhou 215006, People's Republic of China
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  • Shen-Zhou Lu,

    1. School of Materials Engineering, Suzhou University, Suzhou 215006, People's Republic of China
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  • Xue-Guang Zhang

    1. Institute of Medical Biotechnology, Suzhou University; Jiangsu Province Key Laboratory of Stem Cell, Suzhou 215007, People's Republic of China
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Department of Immunology, Binzhou Medical college, Shandong Province, Yantai 264003, People's Republic of China. Tel.: +86 535 6913454. E-mail addresses: xiyingluan@yahoo.com.cn

Abstract

We reported previously that regenerated Antheraea pernyi silk fibroin (A. pernyi SF) could support the attachment and growth of human bone marrow mesenchymal stem cells (hBMSCs). In this work, the immunosupressive effects of hBMSCs cultured on the A. pernyi SF films on T-cells were investigated in vitro. The production of IL-6, CD80, CD86 and HLA-DR by the hBMSCs was also observed. The study showed that hBMSCs cultured on the regenerated A. pernyi SF films still kept their immunosupression on T-cell proliferation and IL-2 secretion. Moreover, regenerated A. pernyi SF like regenerated Bombyx mori SF and collagen did not elicit T-cell proliferation but it could support the expression of IL-6 and surface antigen of hBMSCs. Regenerated A. pernyi SF can maintain the function of hBMSCs in immunomodulation and cytokines production, which has the potential utility of hBMSCs combined with A. pernyi SF in tissue replacement and repair.

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