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Effects of an endothelial cell-conditioned medium on the hematopoietic and endothelial differentiation of embryonic stem cells

Authors

  • Xuan Sun,

    Corresponding author
    1. Institute of Reproductive and Stem Cell Engineering, Central South University, 86 Xiangya Road, Changsha 410078, China
    2. National Engineering and Research Center of Human Stem Cell, Changsha 410078, China
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  • Lamei Cheng,

    1. Institute of Reproductive and Stem Cell Engineering, Central South University, 86 Xiangya Road, Changsha 410078, China
    2. National Engineering and Research Center of Human Stem Cell, Changsha 410078, China
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  • Huaxin Duan,

    1. The People's Hospital of Hunan Province, Changsha 410005, China
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  • Guangxiu Lu

    Corresponding author
    1. Institute of Reproductive and Stem Cell Engineering, Central South University, 86 Xiangya Road, Changsha 410078, China
    2. National Engineering and Research Center of Human Stem Cell, Changsha 410078, China
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Institute of Reproductive and Stem Cell Engineering, Central South University, 86 Xiangya Road, Changsha 410078, China. Tel.: +86 731 2355100 8401; fax: +86 731 4497661. E-mail addresses: lugxdirector@yahoo.com.cn

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Abstract

We have examined the effect of mouse bone marrow endothelial cell-conditioned medium (mEC-CM) on hematopoietic and endothelial differentiation of mouse embryonic stem cells (mESCs). mEC-CM can efficiently promote the differentiation of mESCs into Flk+ cells and hematopoietic colony-forming cells. mEC-CM proved to be as potent as a cytokine cocktail comprised of VEGF, bFGF, IGF and EGF. After inducing mESCs with mEC-CM, cobblestone-like cells were mechanically selected and identified which had the ability to incorporate DiI-Ac-LDL. DiI-Ac-LDL-positive cells were endothelial-like cells due to their expression of CD31 and Flk1, ability to bind to UEA1 and capacity to form capillary-like tube structures on matrigel. In conclusion, mEC-CM can efficiently promote the differentiation of mESCs into endothelial cells and hematopoietic colony-forming cells. The differentiated endothelial-like cells can be isolated by using DiI-Ac-LDL labeling and mechanical selection.

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