These authors contributed equally to this work.
Silencing of calpain expression reduces the metastatic potential of human osteosarcoma cells
Version of Record online: 2 JAN 2013
2009 International Federation for Cell Biology
Cell Biology International
Volume 33, Issue 12, pages 1263–1267, December 2009
How to Cite
Fan, D.-G., Dai, J.-Y., Tang, J., Wu, M.-M., Sun, S.-G., Jiang, J.-L. and Fan, Q.-Y. (2009), Silencing of calpain expression reduces the metastatic potential of human osteosarcoma cells. Cell Biology International, 33: 1263–1267. doi: 10.1016/j.cellbi.2009.08.014
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Received 3 April 2009; revised 2 June 2009; accepted 27 August 2009
- Matrix metalloproteinase;
Osteosarcoma, the most common primary bone tumor in young adults, is characterized by local invasion and distant metastasis. But detailed mechanisms of tumorigenicity and metastasis of osteosarcoma are not well known. We report the involvement of calpains, a family of calcium-activated, cysteine proteases, in the invasive and metastatic processes of human osteosarcoma cells. By using siRNA treatment, the expression of μ- and m-calpains were downregulated in human Saos-2 osteosarcoma cells. Both the adhesive and invasive potentials were significantly attenuated in calpain siRNA-transfected human Saos-2 osteosarcoma cells. MMPs are the main factors involved in malignant tumor invasion and metastasis. siRNA of calpains also significantly inhibited the secretion of MMP-2 in Saos-2 cells. These results suggest that μ- and m-calpains are important in the invasion and metastasis of human osteosarcoma cells, and calpains might be targeted to reduce tumor progression.