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Silencing of calpain expression reduces the metastatic potential of human osteosarcoma cells

Authors

  • De-Gang Fan,

    1. Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710032, People's Republic of China
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    • These authors contributed equally to this work.

  • Jing-Yao Dai,

    1. Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, People's Republic of China
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    • These authors contributed equally to this work.

  • Juan Tang,

    1. Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, the Fourth Military Medical University, 17 West Changle Road, Xi'an 710032, People's Republic of China
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  • Ming-Mei Wu,

    1. Institute of Neuroscience of Chinese PLA, the Fourth Military Medical University, Xi'an 710032, People's Republic of China
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  • Si-Guo Sun,

    1. Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710032, People's Republic of China
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  • Jian-Li Jiang,

    Corresponding author
    1. Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, the Fourth Military Medical University, 17 West Changle Road, Xi'an 710032, People's Republic of China
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  • Qing-Yu Fan

    Corresponding author
    1. Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710032, People's Republic of China
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Tel.: +86 29 84774547; fax: +86 29 83293906. E-mail addresses: jiangjl@fmmu.edu.cn

E-mail addresses: bonetm@fmmu.edu.cn

Abstract

Osteosarcoma, the most common primary bone tumor in young adults, is characterized by local invasion and distant metastasis. But detailed mechanisms of tumorigenicity and metastasis of osteosarcoma are not well known. We report the involvement of calpains, a family of calcium-activated, cysteine proteases, in the invasive and metastatic processes of human osteosarcoma cells. By using siRNA treatment, the expression of μ- and m-calpains were downregulated in human Saos-2 osteosarcoma cells. Both the adhesive and invasive potentials were significantly attenuated in calpain siRNA-transfected human Saos-2 osteosarcoma cells. MMPs are the main factors involved in malignant tumor invasion and metastasis. siRNA of calpains also significantly inhibited the secretion of MMP-2 in Saos-2 cells. These results suggest that μ- and m-calpains are important in the invasion and metastasis of human osteosarcoma cells, and calpains might be targeted to reduce tumor progression.

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