Antarctic bacteria inhibit growth of food-borne microorganisms at low temperatures

Authors

  • Andrea O'Brien,

    1. Microbiology Research Group, Faculty of Health and Human Sciences, Thames Valley University, 32–38 Uxbridge Road Ealing, London W5 2BS, UK
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  • Richard Sharp,

    1. Microbiology Research Group, Faculty of Health and Human Sciences, Thames Valley University, 32–38 Uxbridge Road Ealing, London W5 2BS, UK
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  • Nicholas J. Russell,

    1. Department of Agricultural Sciences, Imperial College London, Wye Campus, Ashford, Kent TN25 5AH, UK
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  • Sibel Roller

    Corresponding author
    1. Microbiology Research Group, Faculty of Health and Human Sciences, Thames Valley University, 32–38 Uxbridge Road Ealing, London W5 2BS, UK
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*Corresponding author. Tel.: +44-20-8280-5108; fax: +44-20-8280-5289. Email address:sibel.roller@tvu.ac.uk

Abstract

The aim of this study was to identify Antarctic microorganisms with the ability to produce cold-active antimicrobial compounds with potential for use in chilled food preservation. Colonies (4496) were isolated from 12 Antarctic soil samples and tested against Listeria innocua, Pseudomonas fragi and Brochothrix thermosphacta. Thirteen bacteria were confirmed as being growth-inhibitor producers (detection rate 0.29%). When tested against a wider spectrum of eight target organisms, some of the isolates also inhibited the growth of L. monocytogenes and Staphylococcus aureus. Six inhibitor producers were psychrotrophic (growth optima between 18 and 24 °C), halotolerant (up to 10% NaCl) and catalase-positive; all but one were Gram-positive and oxidase-positive. The inhibitors produced by four bacteria were sensitive to proteases, suggesting a proteinaceous nature. Four of the inhibitor–producers were shown to be species of Arthrobacter, Planococcus and Pseudomonas on the basis of their 16S rRNA gene sequences and fatty acid compositions. It was concluded that Antarctic soils represent an untapped reservoir of novel, cold-active antimicrobial-producers.

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