Authors contributed equally to manuscript.
Diversity of methanotroph communities in a basalt aquifer
Article first published online: 5 JAN 2006
FEMS Microbiology Ecology
Volume 48, Issue 3, pages 333–344, June 2004
How to Cite
Newby, D.T., Reed, D.W., Petzke, L.M., Igoe, A.L., Delwiche, M.E., Roberto, F.F., McKinley, J.P., Whiticar, M.J. and Colwell, F.S. (2004), Diversity of methanotroph communities in a basalt aquifer. FEMS Microbiology Ecology, 48: 333–344. doi: 10.1016/j.femsec.2004.02.001
- Issue published online: 5 JAN 2006
- Article first published online: 5 JAN 2006
- Received 12 September 2003, Revised 17 December 2003, Accepted 16 February 2004
- Sequence diversity;
- Methane monooxygenase;
Methanotrophic bacteria play an important role in global cycling of carbon and co-metabolism of contaminants. Methanotrophs from pristine regions of the Snake River Plain Aquifer (SRPA; Idaho, USA) were studied in order to gain insight into the native groundwater communities' genetic potential to carry out TCE co-metabolism. Wells were selected that were proximal to a TCE plume believed to be undergoing natural attenuation. Methane concentrations ranged from 1 to >1000 nM. Carbon isotope ratios and diversity data together suggest that the SRPA contains active communities of methanotrophs that oxidize microbially produced methane. Microorganisms removed from groundwater by filtration were used as inocula for enrichments or frozen immediately and DNA was subsequently extracted for molecular characterization. Primers that specifically target methanotroph 16S rRNA genes or genes that code for subunits of soluble or particulate methane monooxygenase, mmoX and pmoA, respectively, were used to characterize the indigenous methanotrophs via PCR, cloning, RFLP analysis, and sequencing. Type I methanotroph clones aligned with Methylomonas, Methylocaldum, and Methylobacter sequences and a distinct 16S rRNA phylogenetic lineage grouped near Methylobacter. The majority of clone sequences in type II methanotroph 16S rRNA, pmoA, and mmoX gene libraries grouped closely with sequences in the Methylocystis genus. A subset of the type II methanotroph clones from the aquifer had sequences that aligned most closely to Methylosinus trichosporium OB3b and Methylocystis spp., known TCE-co-metabolizing methanotrophs.