• [1]
    Madison, L.L., Huisman, G.W. (1999) Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63, 2153.
  • [2]
    Rehm, B.H.A. Polyester synthases: natural catalysts for plastics. Biochem. J. 2003, 376.
  • [3]
    Steinbüchel, A., Schlegel, H.G. (1991) Physiology and molecular genetics of poly(β-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus. Mol. Microbiol. 5, 535542.
  • [4]
    Ostle, A.G., Holt, J.G. (1982) Nile blue a as a fluorescent stain for poly-β-hydroxybutyrate. Appl. Environ. Microbiol. 44, 238241.
  • [5]
    Spiekermann, P., Rehm, B.H.A., Kalscheuer, R., Baumeister, D., Steinbüchel, A. (1999) A sensitive, viable-colony staining method using nile red for direct screening of bacteria that accumulate polyhydroxyalkanoate acids and other lipid storage compounds. Arch. Microbiol. 171, 7380.
  • [6]
    Kranz, R.G., Gabbert, K.K., Madigan, M.T. (1997) Positive selection systems for discovery of novel polyester biosynthesis genes based on fatty acid detoxification. Appl. Environ. Microbiol. 63, 30103013.
  • [7]
    Willis, L.B., Walker, G.C. (1998) The phbC (poly-β-hydroxybutyrate synthase) gene of Rhizobium (Sinorhizobium) meliloti and characterization of phbC mutants. Can. J. Microbiol. 44, 554564.
  • [8]
    Povolo, S., Tombolini, R., Morea, A., Anderson, A.J., Casella, S., Nuti, M.P. (1994) Isolation and characterization of mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrate (PHB). Can. J. Microbiol. 40, 823829.
  • [9]
    Cai, G.-Q., Driscoll, B.T., Charles, T.C. (2000) Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates. J. Bacteriol. 182, 21132118.
  • [10]
    Charles, T.C., Cai, G.-Q., Aneja, P. (1997) Megaplasmid and chromosomal loci for the PHB degradation pathway in Rhizobium (Sinorhizobium) meliloti. Genetics 146, 12111220.
  • [11]
    Charles, T.C., Finan, T.M. (1991) Analysis of a 1600-kilobase Rhizobium meliloti megaplasmid using defined deletions generated in vivo. Genetics 127, 520.
  • [12]
    Charles, T.C., Finan, T.M. (1990) Genetic map of Rhizobium meliloti megaplasmid pRmeSU47b. J. Bacteriol. 172, 24692476.
  • [13]
    Friedman, A.M., Long, S.R., Brown, S.E., Buikema, W.J., Ausubel, F.M. (1982) Construction of a broad host range cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18, 289296.
  • [14]
    Green, L.S., Emerich, D.W. (1997) Bradyrhizobium japonicum does not require α-ketoglutarate dehydrogenase for growth on succinate or malate. J. Bacteriol. 179, 194201.
  • [15]
    Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. (1997) Current Protocols in Molecular Biology. John Wiley & Sons, New York.
  • [16]
    Haywood, G.W., Anderson, A.J., Chu, L., Dawes, E.A. (1988) The role of NADH- and NADPH-linked acetoacetyl-CoA reductases in the poly-3-hydroxybutyrate synthesizing organism Alcaligenes eutrophus. FEMS Microbiol. Lett. 52, 259264.
  • [17]
    Tombolini, R., Povolo, S., Buson, A., Squartini, A., Nuti, M.P. (1995) Poly-β-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti41. Microbiology 141, 25532559.
  • [18]
    Law, J.H., Slepecky, R.A. (1961) Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82, 3336.
  • [19]
    Frey, J., Krisch, H.M. (1985) Ω mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36, 143150.
  • [20]
    Kranz, R.G., Gabbert, K.K., Locke, T.A., Madigan, M.T. (1997) Polyhydroxyalkanoate production in Rhodobacter capsulatus: genes, mutants, expression, and physiology. Appl. Environ. Microbiol. 63, 30033009.
  • [21]
    Fales, L., Kryszak, L., Zeilstra-Ryalls (2001) Control of hemA expression in Rhodobacter sphaeroides 2.4.1: effect of a transposon insertion in the hbdA gene. J. Bacteriol. 183, 15681576.
  • [22]
    Mandon, K., Michel-Reydellet, N., Encarnación, S., Kaminski, P.A., Leija, A., Cevallos, M.A., Elmerich, C., Mora, J. (1998) Poly-β-hydroxybutyrate turnover in Azorhizobium caulinodans is required for growth and affects nifA expression. J. Bacteriol. 180, 50705076.
  • [23]
    Cevallos, M.A., Encarnación, S., Leija, A., Mora, Y., Mora, J. (1996) Genetic and physiological characterization of a Rhizobium etli mutant strain unable to synthesize poly-β-hydroxybutyrate. J. Bacteriol. 178, 16461654.
  • [24]
    Kadouri, D., Burdman, S., Jurkevitch, E., Okon, Y. (2002) Identification and isolation of genes involved in poly-β-hydroxybutyrate biosynthesis in Azospirillum brasilense and characterization of a phbC mutant. Appl. Environ. Microbiol. 68, 29432949.
  • [25]
    Castañeda, M., Guzmán, J., Moreno, S., Espín, G. (2000) The GacS sensor kinase regulates alginate and poly-β-hydroxybutyrate production in Azotobacter vinelandii. J. Bacteriol. 182, 26242628.
  • [26]
    Pellock, B.J., Teplitski, M., Boinay, R.P., Bauer, W.D., Walker, G.C. (2002) A LuxR homolog controls production of symbiotically active extracellular polysaccharide II by Sinorhizobium meliloti. J. Bacteriol. 184, 50675076.
  • [27]
    Kaneko, T., Nakamura, Y., Sato, S., Minamisawa, K., Uchiumi, T., Sasamoto, S., Watanabe, A., Idesawa, K., Iriguchi, M., Kawashima, K., Kohara, M., Matsumoto, M., Shimpo, S., Tsuruoka, H., Wada, T., Yamada, M., Tabata, S. (2002) Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Res. 9, 189197.
  • [28]
    Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, Z., Miller, W., Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic. Acids Res. 25, 33893402.
  • [29]
    Thompson, J.D., Higgins, D.G., Gibson, T.J. (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 46734680.
  • [30]
    Larimer, F.W., Chain, P., Hauser, L., Lamerdin, J., Malfatti, S., Do, L., Land, M.L., Pelletier, D.A., Beatty, J.T., Lang, A.S., Tabita, F.R., Gibson, J.L., Hanson, T.E., Bobst, C., Torres y Torres, J.L., Peres, C., Harrison, F.H., Gibson, J., Harwood, C.S. (2004) Complete genome sequence of the metabolically versatile bacterium Rhodopseudomonas palustris. Nature Biotech. 22, 5561.
  • [31]
    Peoples, O.P., Sinskey, A.J. (1989) Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264, 1529815303.
  • [32]
    McCool, G.J., Cannon, M.C. (1999) Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium. J. Bacteriol. 181, 585592.
  • [33]
    McCool, G.J., Cannon, M.C. (2001) PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium. J. Bacteriol. 183, 42354243.
  • [34]
    Meade, H.M., Long, S.R., Ruvkun, G.B., Brown, S.E., Ausubel, F.M. (1982) Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon mutagenesis. J. Bacteriol. 149, 114122.
  • [35]
    Finan, T.M., Hartwieg, E.K., LeMieux, K., Bergman, K., Walker, G.C., Signer, E.R. (1984) General transduction in Rhizobium meliloti. J. Bacteriol. 159, 120124.
  • [36]
    Aneja, P., Dziak, R., Cai, G.-Q., Charles, T.C. (2002) Identification of an acetoacetyl coenzyme A synthetase-dependent pathway for utilization of L-(+)-3-hydroxybutyrate in Sinorhizobium meliloti. J. Bacteriol. 184, 15711577.
  • [37]
    Leigh, J.A., Signer, E., Walker, G. (1985) Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc. Natl. Acad. Sci. USA 82, 62316235.
  • [38]
    Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557580.
  • [39]
    Finan, T.M., Kunkel, B., DeVos, G.F., Signer, E.R. (1986) Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J. Bacteriol. 167, 6672.
  • [40]
    Quandt, J., Hynes, M.F. (1993) Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127, 1521.
  • [41]
    Vieira, J., Messing, J. (1982) The pUC plasmids, and M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19, 259268.
  • [42]
    Yanisch-Perron, C., Vieira, J., Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103119.
  • [43]
    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic. Acids Res. 25, 48764882.
  • [44]
    Felsenstein, J. (1989) PHYLIP – phylogeny inference package (Version 3.2). Cladistics 5, 164166.