You have full text access to this OnlineOpen article

Differences in gene expression of the ciliate Paramecium caudatum caused by endonuclear symbiosis with Holospora obtusa, revealed using differential display reverse transcribed PCR

  1. Yoshimitsu Nakamura,
  2. Masako Aki,
  3. Tomonori Aikawa,
  4. Manabu Hori,
  5. Masahiro Fujishima*

Article first published online: 9 JAN 2006

DOI: 10.1016/j.femsle.2004.09.036

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 240, Issue 2, pages 209–213, November 2004

Additional Information

How to Cite

Nakamura, Y., Aki, M., Aikawa, T., Hori, M. and Fujishima, M. (2004), Differences in gene expression of the ciliate Paramecium caudatum caused by endonuclear symbiosis with Holospora obtusa, revealed using differential display reverse transcribed PCR. FEMS Microbiology Letters, 240: 209–213. doi: 10.1016/j.femsle.2004.09.036

Author Information

  1. Faculty of Science, Biological Institute, Yamaguchi University, 1677-1, Yamaguchi 753-8512, Japan

* *Corresponding author. Tel./fax: +81 83 933 5712, E-mail address: fujishim@yamaguchi-u.ac.jp

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 15 July 2004, Revised 31 August 2004, Accepted 22 September 2004

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (288K)

Keywords:

  • Holospora obtusa;
  • Paramecium caudatum;
  • Differential display reverse transcribed PCR;
  • Phosphatidylinositol-4-phosphate 5-kinase;
  • Small nuclear ribonucleoprotein;
  • Pyruvate dehydrogenase E1 α subunit

Abstract

We identified six genes of Paramecium caudatum, which differentially expressed in Holospora obtusa-bearing and H. obtusa-free cells using differential display reverse transcribed PCR (DDRT-PCR). Northern blot analyses revealed that two of the genes, CA10-3 and CA20-2, were expressed extensively in the H. obtusa-free cell, while the other four, AS16-1, CS14, CS21 and CA17-1, were expressed more in the H. obtusa-bearing cell. Putative amino acid sequences of CA10-3, AS16-1 and CA17-1 showed high homologies with known genes related to intracellular signaling, transcription and aerobic metabolism. CS14 and CS21 also showed homologies with some genes whose products are still functionally unknown, but CA20-2 encoded a novel protein. We show in this study that H. obtusa alters multiple gene expression of the host after establishing endosymbiosis.

View Full Article (HTML) Get PDF (288K)