Chemiluminescence of enterococci isolates from freshwater

Authors

  • Philippe Andre,

    Corresponding author
    1. U.F.R. des Sciences Pharmaceutiques, UE-3429, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France
      *Corresponding author. Tel.: +33 03 90 24 41 43; fax: +33 00 90 24 42 86, E-mail address: andre@pharma.u-strasbg.fr
    Search for more papers by this author
    • 1

      New address: U.F.R. des Sciences Pharmaceutiques, UMR CNRS 7034, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France.

  • Catherine Metzger,

    1. U.F.R. des Sciences Pharmaceutiques, UE-3429, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France
    Search for more papers by this author
  • Sophie Petey,

    1. U.F.R. des Sciences Pharmaceutiques, UE-3429, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France
    Search for more papers by this author
  • Daniel Muller,

    1. Laboratoire de Dynamique, Évolution et Expression des génomes de micro-organismes, FRE 2326 ULP/CNRS 28, rue Goethe, 67083 Strasbourg Cedex, France
    Search for more papers by this author
  • Dominique J.-M. Vidon

    1. U.F.R. des Sciences Pharmaceutiques, UE-3429, 74 route du Rhin, B.P. 24, 67401 Illkirch Cedex, France
    Search for more papers by this author

  • Edited by A. Oren

*Corresponding author. Tel.: +33 03 90 24 41 43; fax: +33 00 90 24 42 86, E-mail address: andre@pharma.u-strasbg.fr

Abstract

All Enterococcus spp., isolated from environmental water samples (n= 81), emitted a high chemiluminescence signal in the presence of luminol (10−2 M). Kinetic studies of chemiluminescence show a close correlation between chemiluminescence and growth curves during the exponential phase, with a maximum chemiluminescence reached just before bacterial growth entered in the stationary phase. On the other hand, genera closely related to Enterococcus such as Streptococcus or Lactococcus produced a very weak chemiluminescent signal. Chemiluminescence of enterococci could therefore offer a rapid test, in aiding the identification of the genus Enterococcus and in the survey of the microbiological quality of water supplies.

Ancillary