• [1]
    Karmali, M.A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2, 538.
  • [2]
    Nataro, J.P., Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11, 142201.
  • [3]
    Blanco, M., Blanco, J.E., Mora, A., Rey, J., Alonso, J.M., Hermoso, M., Alonso, M.P., Dahbi, G., González, E.A., Bernardez, M.I., Blanco, J. (2003) Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from healthy sheep in Spain. J. Clin. Microbiol. 41, 13511365.
  • [4]
    Blanco, M., Blanco, J.E., Mora, A., Dahbi, G., Alonso, M.P., González, E.A., Bernardez, M.I., Blanco, J. (2004) Serotypes, virulence genes and intimin types of Shiga toxin (Verotoxin)-producing Escherichia coli isolates from cattle in Spain: identification of a new intimin variant gene (eae-ξ). J. Clin. Microbiol. 42, 645651.
  • [5]
    Nakao, H., Takeda, T. (2000) Escherichia coli Shiga toxin. J. Nat. Toxins 9, 299313.
  • [6]
    Brett, K.N., Hornitzky, M.A., Bettelheim, K.A., Walker, M.J., Djordjevic, S.P. (2003) Bovine non-O157 Shiga toxin 2-containing Escherichia coli isolates commonly possess stx2-EDL933 and/or stx2vhb subtypes. J. Clin. Microbiol. 41, 27162722.
  • [7]
    Elliott, S.J., Wainwright, L.A., McDaniel, T.K., Jarvis, K.G., Deng, Y.K., Lai, L.C., McNamara, B.P., Donnenberg, M.S., Kaper, J.B. (1998) The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol. Microbiol. 28, 14.
  • [8]
    Schmidt, H., Beutin, L., Karch, H. (1995) Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933. Infect. Immun. 63, 10551061.
  • [9]
    Paton, J.C., Paton, A.W. (1998) Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11, 450479.
  • [10]
    Chalmers, R.M., Aird, H., Bolton, F.J. Waterborne Escherichia coli O157. Symp. Ser. Soc. Appl. Microbiol., 2000, 124S–132S
  • [11]
    Blanch, A.R., García-Aljaro, C., Muniesa, M., Jofre, J. (2003) Detection, enumeration and isolation of strains carrying the stx2 gene from urban sewage. Water Sci. Technol. 47, 109116.
  • [12]
    Clesceri, L.S., Greenberg, A.E., Eaton, A.D., Eds. Standard methods for the examination of water and wastewater. 20th ed. 1998, American Public Health Association, Washington, DC
  • [13]
    García-Aljaro, C., Muniesa, M., Jofre, J., Blanch, A.R. (2004) Prevalence of the stx2 gene in coliform populations from aquatic environments. Appl. Environ. Microbiol. 70, 35353540.
  • [14]
    Muniesa, M., De Simon, M., Prats, G., Ferrer, D., Panella, H., Jofre, J. (2003) Shiga toxin 2-converting bacteriophages associated with clonal variability in Escherichia coli O157:H7 strains of human origin isolated from a single outbreak. Infect. Immun. 71, 45544562.
  • [15]
    Blanch, A.R., Caplin, J.L., Iversen, A., Kühn, I., Manero, A., Taylor, H.D., Vilanova, X. (2004) Comparison of enterococcal populations related to urban and hospital wastewater in various climatic and geographic European regions. J. Appl. Microbiol. 94, 9941002.
  • [16]
    Hitchcock, P.J., Brown, T.M (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154, 269277.
  • [17]
    Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.
  • [18]
    Tsai, C.M., Frasch, C.E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119, 115119.
  • [19]
    Guinée, P.A.M., Jansen, W.H., Wadström, T., Sellwood, R. (1981) Escherichia coli associated with neonatal diarrhoea in piglets and calves. Curr. Top Vet. Anim. Sci. 13, 126162.
  • [20]
    Khakhria, R., Duck, D., Lior, H. (1990) Extended phage-typing scheme for Escherichia coli O157:H7. Epidemiol. Infect. 105, 511520.
  • [21]
    Park, C.H., Kim, H.J., Hixon, D.L., Bubert, A. (2003) Evaluation of the duopath verotoxin test for detection of shiga toxins in cultures of human stools. J. Clin. Microbiol. 41, 26502653.
  • [22]
    Stewart, D.S., Tortorello, M.L., Gendel, S.M. (1998) Evaluation of DNA preparation techniques for detection of the SLT-1 gene of Escherichia coli O157:H7 in bovine faeces using the polymerase chain reaction. Lett. Appl. Microbiol. 26, 9397.
  • [23]
    Sambrook, J., Russell, D.W. (2001) Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  • [24]
    Corpet, F. (1998) Multiple sequence alignment with hierarchical clustering. Nucl. Acid Res. 16, 1088110890.
  • [25]
    Rajkowski, K.T., Rice, E.W. (1999) Recovery and survival of Escherichia coli O157:H7 in reconditioned pork-processing wastewater. J. Food. Prot. 62, 731734.
  • [26]
    Tschäpe, H., Prager, R., Streckel, W., Fruth, A., Tietze, E., Bohme, G. (1995) Verotoxinogenic Citrobacter freundii associated with severe gastroenteritis and cases of haemolytic uraemic syndrome in a nursery school green butter as the infection source. Epidemiol. Infect. 114, 441450.
  • [27]
    World Health Organization Scientific Working Group (1998) Zoonotic non-O157 Shiga toxin-producing Escherichia coli (STEC), 23–26 June 1998, Berlin, Germany, pp. 1–30. W.H.O./CSR/APH/98.8. World Health Organization, Geneva, Switzerland
  • [28]
    Beutin, L., Krause, G., Zimmermann, S., Kaulfuss, S., Gleier, K. (2004) Charaterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42, 10991108.
  • [29]
    Blanco, J.E., Blanco, M., Alonso, P., Mora, A., Dahbi, G., Coira, M.A., Blanco, J. (2004) Serotypes, virulence genes, and intimin types of shiga toxin (verotoxin)-producing Escherichia coli isolates from human patients, prevalence in Lugo, Spain, from 1992 through 1999. J. Clin. Microbiol. 42, 311319.
  • [30]
    Saari, M., Cheasty, T., Leino, K., Siitonen, A. (2001) Phage types and genotypes of Shiga toxin-producing Escherichia coli O157 in Finland. J. Clin. Microbiol. 39, 11401143.
  • [31]
    Mora, A., Blanco, M., Blanco, J.E., Alonso, M.P., Dhabi, G., Thompson-Carter, F., Usera, M.A., Bartolome, R., Prats, G., Blanco, J. (2004) Phage types and genotypes of human and animal shiga toxin-producing Escherichia coli O157:H7 in Spain. Identification of two predominating phage types (PT2 and PT8) which differed in their motility, stx-genotypes, PFGE patterns and the severity of the illness they caused. J. Clin. Microbiol. 42, 40074015.
  • [32]
    Boerlin, P., McEwen, S.A., Boerlin-Petzold, F., Wilson, J.B., Johnson, R.P., Gyles, C.L. (1999) Associations between virulence factors of shiga toxin-producing Escherichia coli and disease in humans. J. Clin. Microbiol. 37, 497503.
  • [33]
    Siegler, R.L., Obrig, T.G., Pysher, T.J., Tesh, V.L., Denkers, N.D., Taylor, F.B. (2003) Response to Shiga toxin 1 and 2 in a baboon model of hemolytic uremic syndrome. Pediatr. Nephrol. 18, 9296.
  • [34]
    Ramachandran, V., Hornitzky, M.A., Bettelheim, K.A., Walker, M.J., Djordjevic, V. (2001) The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type. J. Clin. Microbiol. 39, 19321937.
  • [35]
    Marques, L.R.M., Peiris, J.S., Cryz, S.J., O’ Brien, A.D. (1987) Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin II. FEMS Microbiol. Lett. 44, 3338.
  • [36]
    Morabito, S., Dell' Omo, G., Agrimi, U., Schmidt, H., Karch, H., Cheasty, T., Caprioli, A. (2001) Detection and characterization of Shiga toxin-producing Escherichia coli in feral pigeons. Vet. Microbiol. 82, 275283.
  • [37]
    Leung, P.H., Peiris, J.S., Ng, W.W., Robins-Browne, V., Bettelheim, K.A., Yam, W.C. (2003) A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli. Appl. Environ. Microbiol. 69, 75497553.
  • [38]
    Kaper, J.B., Elliott, S., Sperandio, V., Perna, N.T., Mayhew, G.F., Blattner, F.R. (1998) Attaching and effacing intestinal histopathology and the locus of enterocyte effacement. In: Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains (Kaper, J.B., O’ Brien, A.D., Eds.), pp.163–182 American Society for Microbiology, Washington, DC.
  • [39]
    Mühldorfer, I., Hacker, J., Keusch, G.T., Acheson, D.W., Tschäpe, H., Kane, A.V., Rittler, A., Olschlager, T., Donohue-Rolte, A. (1996) Regulation of the Shiga-like toxin II operon in Escherichia coli. Infect. Immun. 64, 495502.
  • [40]
    Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557580.
  • [41]
    Bertin, Y., Boukhors, K., Pradel, N., Livrelli, V., Martin, C. (2001) Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors. J. Clin. Microbiol. 39, 30603065.
  • [42]
    Wells, J.G., Davis, B.R., Wachsmuth, I.K., Riley, L.W., Remis, R.S., Sokolow, R., Morris, G.K. (1983) Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18, 512520.
  • [43]
    Jenkins, C., Perry, N.T., Cheasty, T., Shaw, D.J., Frankel, G., Dougan, G., Gunn, G.J., Smith, H.R., Paton, A.W., Paton, J.C. (2003) Distribution of the saa gene in strains of Shiga toxin-producing Escherichia coli of human and bovine origins. J. Clin. Microbiol. 41, 17751778.
  • [44]
    Muniesa, M., Jofre, J. (1998) Abundance in sewage of bacteriophages that infect Escherichia coli O157:H7 and that carry the Shiga toxin 2 gene. Appl. Environ. Microbiol. 64, 24432448.
  • [45]
    Wang, G., Clark, C.G., Rodgers, F.G. (2002) Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7 serotype and components of the type 2 Shiga toxin family by multiplex PCR. J. Clin. Microbiol. 40, 36133619.
  • [46]
    Paton, A.W., Paton, J.C. (2002) Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA and saa. J. Clin. Microbiol. 40, 271274.
  • [47]
    Desmarchelier, P.M., Bilge, S.S., Fegan, N., Mills, L. J.C. Vary Jr. Tarr, P.I. (1998) A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J. Clin. Microbiol. 36, 18011804.
  • [48]
    Gannon, V.P., D'Souza, S., Graham, T., King, R.K., Rahn, K., Read, S. (1997) Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J. Clin. Microbiol. 35, 656662.
  • [49]
    Weisburg, W.G., Barns, S.M., Pelletier, D.A., Lane, D.J. (1991) 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697703.