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PCR-generated artefact from 16S rRNA gene-specific primers

  1. Catherine A. Osborne,
  2. Maja Galic,
  3. Parveen Sangwan,
  4. Peter H. Janssen*

Article first published online: 9 JAN 2006

DOI: 10.1016/j.femsle.2005.05.043

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 248, Issue 2, pages 183–187, July 2005

Additional Information

How to Cite

Osborne, C. A., Galic, M., Sangwan, P. and Janssen, P. H. (2005), PCR-generated artefact from 16S rRNA gene-specific primers. FEMS Microbiology Letters, 248: 183–187. doi: 10.1016/j.femsle.2005.05.043

Author Information

  1. Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia

* *Corresponding author. Tel.: +61 3 8344 5706; fax: +61 3 9347 1540., E-mail address: pjanssen@unimelb.edu.au

  1. Edited by C. Edwards

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 1 March 2005, Revised 12 May 2005, Accepted 19 May 2005

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Keywords:

  • PCR artefact;
  • 16S rRNA gene PCR;
  • 16S rRNA gene primers;
  • Primer concatamer

Abstract

Artefacts consisting of concatenated oligonucleotide primer sequences were generated during sub-optimally performing polymerase chain reaction amplification of bacterial 16S rRNA genes using a commonly employed primer pair. These artefacts were observed during amplification for terminal restriction fragment length polymorphism analyses of complex microbial communities, and after amplification from DNA from a microbial culture. Similar repetitive motifs were found in gene sequences deposited in GenBank. The artefact can be avoided by using different primers for the amplification reaction.

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