These authors contributed equally to this work.
Accumulation of S-adenosylmethionine induced oligopeptide transporters including BldK to regulate differentiation events in Streptomyces coelicolor M145
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 249, Issue 2, pages 199–206, August 2005
How to Cite
Park, H.-S., Shin, S.-K., Yang, Y.-Y., Kwon, H.-J. and Suh, J.-W. (2005), Accumulation of S-adenosylmethionine induced oligopeptide transporters including BldK to regulate differentiation events in Streptomyces coelicolor M145. FEMS Microbiology Letters, 249: 199–206. doi: 10.1016/j.femsle.2005.05.047
Edited by A. Klier
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 25 March 2005, Revised 22 May 2005, Accepted 26 May 2005
- Two-dimensional gel electrophoresis;
- Regulation of differentiation
S-Adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was recently found to be involved in the regulation of differentiation in streptomycetes. Exogenous SAM, in a quantity as low as 2 μM, enhanced antibiotic production and inhibited morphological development of Streptomyces coelicolor M145. Total protein profiling of S. coelicolor M145 revealed that SAM enhanced the expression of oligopeptide-binding components related to ABC transporters that included BldK, a well-known regulatory factor in S. coelicolor differentiation. A radiolabeled SAM feeding experiment verified that exogenous SAM can be imported into the cell, which is under the control of the bld cascade. This study substantiated that BldK serves as a transducer of the SAM signal and uncovered the possible role of oligopeptide import in the regulation of Streptomyces differentiation, particularly in relation to SAM.