Biofilm microbial community of a thermophilic trickling biofilter used for continuous biohydrogen production

Authors

  • Yeonghee Ahn,

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Guseong-dong, Yuseong-gu, Daejeon 305–701, Republic of Korea
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  • Eun-Jung Park,

    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Guseong-dong, Yuseong-gu, Daejeon 305–701, Republic of Korea
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  • You-Kwan Oh,

    1. Department of Chemical and Biochemical Engineering, Pusan National University, San 30 Jangjeon-dong, Kumjung-ku Pusan 609–735, Republic of Korea
    2. Institute for Environmental Technology and Industry, Pusan National University, San 30 Jangjeon-dong, Kumjung-ku Pusan 609–735, Republic of Korea
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  • Sunghoon Park,

    1. Department of Chemical and Biochemical Engineering, Pusan National University, San 30 Jangjeon-dong, Kumjung-ku Pusan 609–735, Republic of Korea
    2. Institute for Environmental Technology and Industry, Pusan National University, San 30 Jangjeon-dong, Kumjung-ku Pusan 609–735, Republic of Korea
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  • Gordon Webster,

    1. Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, Wales, United Kingdom
    2. Cardiff School of Earth, Ocean and Planetary Sciences, Cardiff University, Main Building, Park Place, Cardiff, CF10 3YE, Wales, United Kingdom
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  • Andrew J. Weightman

    1. Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, Wales, United Kingdom
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  • Edited by E. Baggs

*Corresponding author. Tel.: +82 42 869 3941; fax: +82 42 869 3910., E-mail address: yahn@kaist.ac.kr

Abstract

Molecular methods were employed to investigate the microbial community of a biofilm obtained from a thermophilic trickling biofilter reactor (TBR) that was operated long-term to produce H2. Biomass concentration in the TBR gradually decreased as reactor bed height increased. Despite this difference in biomass concentration, samples from the bottom and middle of the TBR bed revealed similar microbial populations as determined by PCR-DGGE analysis of 16S rRNA genes. Nucleotide sequences of most DGGE bands were affiliated with the classes Clostridia and Bacilli in the phylum Firmicutes, and the most dominant bands showed a high sequence similarity to Thermoanaerobacterium thermosaccharolyticum.

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