Lactobacillus fermentum CRL 722 is able to deliver active α-galactosidase activity in the small intestine of rats

Authors

  • Jean Guy LeBlanc,

    Corresponding author
    1. Centro de Referencia para Lactobacilos (CERELA-CONICET). Chacabuco 145, San Miguel de Tucumán, Tucumán, Argentina (T4000ILC)
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  • Jean-Christophe Piard,

    1. Useful Bacterial Surface Proteins Group, INRA-URLGA, Jouy-en-Josas Cedex, France
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  • Fernando Sesma,

    1. Centro de Referencia para Lactobacilos (CERELA-CONICET). Chacabuco 145, San Miguel de Tucumán, Tucumán, Argentina (T4000ILC)
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  • Graciela Savoy de Giori

    1. Centro de Referencia para Lactobacilos (CERELA-CONICET). Chacabuco 145, San Miguel de Tucumán, Tucumán, Argentina (T4000ILC)
    2. Cátedra de Microbiología Superior, Universidad Nacional de Tucumán (UNT), Tucumán, Argentina (T4000ILC)
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  • Edited by W. Kneifel

*Corresponding author. Tel.: +54 381 431 0465; fax: +54 381 400 5600., E-mail address: leblanc@cerela.org.ar

Abstract

α-galactooligosaccharides (α-GOS) found in legumes such as soybeans can cause gastrointestinal disorders since mammals lack α-galactosidase (α-Gal) in the small intestine which is necessary for their hydrolysis. Lactobacillus fermentum CRL 722 is a lactic acid bacterium (LAB) capable of degrading α-GOS due to its elevated α-Gal activity. When conventional rats were fed live L. fermentum CRL 722 or cell-free extracts of this strain, a short-lived α-Gal activity was detected in the upper gastrointestinal tract. The safety of this LAB was also assessed. L. fermentum CRL 722 could thus be used as a vehicle to safely confer α-Gal in the small intestine of monogastric animal.

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