Differential expression of the chsE gene encoding a chitin synthase of Aspergillus nidulans in response to developmental status and growth conditions

Authors

  • Jeong Im Lee,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Yeong Man Yu,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Yoo Mi Rho,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Bum Chan Park,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Jeong Hwan Choi,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Hee-Moon Park,

    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Pil Jae Maeng

    Corresponding author
    1. Department of Microbiology, School of Bioscience & Biotechnology, Chungnam National University, Daejeon 305–764, Republic of Korea
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  • Edited by M. Jacquet

*Corresponding author. Tel.: +82 42 821 6415; fax: +82 42 822 7367., E-mail address: pjmaeng@cnu.ac.kr

Abstract

Expression of chsE encoding one of the five chitin synthases of Aspergillus nidulans was analyzed. Expression of chsE was moderate in conidiophores, but somewhat weaker in vegetative mycelia. During sexual development, chsE was expressed strongly in young cleistothecia and hülle cells, but little in mature sexual structures. Deletion of chsE caused a significant decrease in the chitin content of the cell wall during early sexual development. Expression of chsE was increased by substituting glucose with lactose or by addition of 0.6 M KCl or NaCl, but affected little by substituting glucose with sodium acetate. Consequently, chsE was shown to have a mode of expression distinct from those of the other chitin synthase genes, chsA, chsB and chsC.

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