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Keywords:

  • Omphalotus;
  • Nonribosomal peptide synthetase;
  • Siderophore;
  • Ferrichrome A;
  • Gene cluster

Abstract

Under iron deprivation Omphalotus olearius was found to produce the hydroxamate siderophore ferrichrome A. A gene cluster consisting of three genes: fso1, a nonribosomal peptide synthetase whose expression is enhanced in the absence of iron; omo1, a l-ornithine-N5-monooxygenase; and ato1, an acyltransferase probably involved in the transfer of the methylglutaconyl residue to N5-hydroxyorinithine was identified. The fso1 sequence is interrupted by 48 introns and its derived protein sequence has a similar structure to the homologous genes of Ustilago maydis and Aspergillus nidulans. This is the first report of a nonribosomal peptide synthetase gene and a biosynthetic gene cluster in homobasidiomycetes.