Drug Research and Medical Biotechnology, Fraunhofer Institute of Toxicology, Experimental Medicine, Nikolai Fuchs Strasse 1, 30625 Hannover, Germany.
Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 249, Issue 2, pages 233–240, August 2005
How to Cite
Battistoni, F., Bartels, D., Kaiser, O., Marie Reamon-Buettner, S., Hurek, T. and Reinhold-Hurek, B. (2005), Physical map of the Azoarcus sp. strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis. FEMS Microbiology Letters, 249: 233–240. doi: 10.1016/j.femsle.2005.06.015
Edited by M.Y. Galperin
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 1 May 2005, Revised 5 June 2005, Accepted 9 June 2005
- BAC library;
- Physical map;
- Genome sequencing;
- Functional genomics
Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the β subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome.