Contributed equally to this work.
Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 250, Issue 2, pages 253–261, September 2005
How to Cite
Dussurget, O., Dumas, E., Archambaud, C., Chafsey, I., Chambon, C., Hébraud, M. and Cossart, P. (2005), Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence. FEMS Microbiology Letters, 250: 253–261. doi: 10.1016/j.femsle.2005.07.015
Edited by M. Mitsuyama
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 30 April 2005, Revised 13 July 2005, Accepted 13 July 2005
In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. We also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of L. monocytogenes. A comparative proteomic analysis revealed an effect of the fri deletion on the levels of listeriolysin O and several stress proteins. Together, our study demonstrates that fri has multiple roles that contribute to Listeria virulence.