• [1]
    Cregg, J.M., Cereghino, J.L., Shi, J., Higgins, D.R. (2000) Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16, 2331.
  • [2]
    Baneyx, F. (1999) Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10, 411421.
  • [3]
    Pedersen, H., Lamsa, M., Hansen, P.K., Frisner, H., Vind, J., Ernst, S., Kongsbak, L., Joergensen, B.R., Beck, T.C., Husum, T.L. and von Ossowski, I. (2001) Microtiter plate based high-throughput screening assays for large numbers of cell populations. WO 2001032844 [Chem. Abstr. 2001, 134, 350243]
  • [4]
    Menzel, R. (1989) A microtiter plate-based system for the semiautomated growth and assay of bacterial cells for beta-galactosidase activity. Anal. Biochem. 181, 4050.
  • [5]
    Duetz, W.A., Ruedi, L., Hermann, R., O'Connor, K., Buchs, J., Witholt, B. (2000) Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Appl. Environ. Microbiol. 66, 26412646.
  • [6]
    Holz, C., Hesse, O., Bolotina, N., Stahl, U., Lang, C. (2002) A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae. Protein Expres. Purif. 25, 372378.
  • [7]
    Arnold, F.H. (1998) Design by directed evolution. Acc. Chem. Res. 31, 125131.
  • [8]
    Walsh, G. (2003) Biopharmaceutical benchmarks – 2003. Nat. Biotechnol. 21, 865878.
  • [9]
    Strobel, R., Bowden, D., Bracey, M., Sullivan, G., Hatfield, C., Jenkins, N., Vinci, V. (2001) High-throughput cultivation of animal cells using shaken microplate techniques. In: Animal Cell Technology: From Target to Market (Lindner-Olsson, E., Chatzissavidou, N., Luellau, E., Eds.), pp.307–312 Kluwer Academic Publishers, Dordrecht, The Netherlands.
  • [10]
    Boettner, M., Prinz, B., Holz, C., Stahl, U., Lang, C. (2002) High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J. Biotechnol. 99, 5162.
  • [11]
    Petrounia, I.P., Arnold, F.H. (2000) Designed evolution of enzymatic properties. Curr. Opin. Biotechnol. 11, 325330.
  • [12]
    Arnold, F.H., Georgiou, G. (2003) Directed Enzyme Evolution: Screening and Selection Methods. Humana Press Inc, Totowa.
  • [13]
    Olsen, M., Iverson, B., Georgiou, G. (2000) High-throughput screening of enzyme libraries. Curr. Opin. Biotechnol. 11, 331337.
  • [14]
    Farinas, E.T., Bulter, T., Arnold, F.H. (2001) Directed enzyme evolution. Curr. Opin. Biotechnol. 12, 545551.
  • [15]
    Lin Cereghino, J., Cregg, J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24, 4566.
  • [16]
    Fröhlich, K.U., Madeo, F. (2003) Genetics of Apoptosis: Regulators and Applications of Yeast Apoptosis. Oxford University Press, Oxford, GB.
  • [17]
    Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidmann, J.G., Smith, J.A., Struhl, K. (2003) Current Protocols in Molecular Biology. Wiley, New York.
  • [18]
    Weis, R., Poechlauer, P., Bona, R., Skranc, W., Luiten, R., Wubbolts, M., Schwab, H., Glieder, A. (2004) Biocatalytic conversion of unnatural substrates by recombinant almond R-HNL isoenzyme 5. J. Mol. Cat. B: Enzymatic 29, 211218.
  • [19]
    Hasslacher, M., Schall, M., Hayn, M., Bona, R., Rumpold, K., Lückl, J., Griengl, H., Kohlwein, S.D., Schwab, H. (1997) High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts. Protein Expres. Purif. 11, 6171.
  • [20]
    Glieder, A., Weis, R., Skranc, W., Pöchlauer, P., Dreveny, I., Majer, S., Wubbolts, M., Schwab, H., Gruber, K. (2003) Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis. Angew. Chem. Int. Ed. Engl. 42, 48154818.
  • [21]
    Madeo, F., Fröhlich, E., Fröhlich, K.U. (1997) A yeast mutant showing diagnostic markers of early and late apoptosis. J. Cell. Biol. 139, 729734.
  • [22]
    Madeo, F., Fröhlich, E., Ligr, M., Grey, M., Sigrist, S.J., Wolf, D.H., Fröhlich, K.U. (1999) Oxygen stress: a regulator of apoptosis in yeast. J. Cell. Biol. 145, 757767.
  • [23]
    Morawski, B., Lin, Z., Cirino, P., Joo, H., Bandara, G., Arnold, F.H. (2000) Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 13, 377384.
  • [24]
    Cino, J. High yield protein production from Pichia pastoris yeast: a protocol for benchtop fermentation. In: New Brunswick Scientific (Edison, N.J., Ed.)
  • [25]
    Hohenblum, H., Borth, N., Mattanovich, D. (2003) Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J. Biotechnol. 102, 281290.
  • [26]
    Duetz, W.A., Witholt, B. (2001) Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square-deepwell microtiter plates. Biochem. Eng. J. 7, 113115.
  • [27]
    Hermann, R., Lehmann, M., Buechs, J. (2003) Characterization of gas–liquid mass transfer phenomena in microtiter plates. Biotechnol. Bioeng. 81, 178186.
  • [28]
    Whittaker, M.M., Whittaker, J.M. (2000) Expression of recombinant galactose oxidase by Pichia pastoris. Protein Expres. Purif. 20, 105111.
  • [29]
    Granot, D., Levine, A., Dor-Hefetz, E. (2003) Sugar-induced apoptosis in yeast cells. FEMS Yeast Res. 4, 713.
  • [30]
    Egli, T. (1982) Regulation of protein synthesis in methylotrophic yeasts: repression of methanol dissimilating enzymes by nitrogen limitation. Arch. Microbiol. 131, 95101.
  • [31]
    Martinet, W., van der Plas, D., Raes, H., Reekmans, R., Contreras, R. (1999) van der Bax-induced cell death in Pichia pastoris. Biotechnol. Lett. 21, 821829.