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Expression of HPV16 E6 oncoprotein increases resistance to several stress conditions in Saccharomyces cerevisiae

  1. Ana Pinto-de-Oliveira1,2,
  2. Dennis McCance3,
  3. Augusto C. de Magalhães-Sant'Ana2,
  4. Joana M. Marques1,2,
  5. Teresa Gonçalves1,2,*

Article first published online: 9 JAN 2006

DOI: 10.1016/j.femsyr.2005.02.002

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 5, Issue 8, pages 777–787, May 2005

Additional Information

How to Cite

Pinto-de-Oliveira, A., McCance, D., de Magalhães-Sant'Ana, A. C., Marques, J. M. and Gonçalves, T. (2005), Expression of HPV16 E6 oncoprotein increases resistance to several stress conditions in Saccharomyces cerevisiae. FEMS Yeast Research, 5: 777–787. doi: 10.1016/j.femsyr.2005.02.002

Author Information

  1. 1

    Centre for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal

  2. 2

    Institute of Microbiology, Faculty of Medicine, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal

  3. 3

    School of Medicine and Dentistry, University of Rochester, 601 Elmwood Avenue, P.O. Box 672, Rochester, NY 14642, USA

* *Corresponding author. Tel.: +351 239 857700; fax: +351 239 823236, E-mail address: tmfog@ci.uc.pt

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 20 May 2004, Revised 26 November 2004, Accepted 3 February 2005

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Keywords:

  • Papillomavirus;
  • Oncoprotein;
  • Yeast;
  • Stress;
  • Pheromone;
  • Caffeine

Abstract

The E6 protein of human papillomavirus type 16 is essential for the oncogenic transformation process induced by these viruses. Here we expressed the E6 protein in Saccharomyces cerevisiae (which lacks p53) in order to determine if E6 interacts with normal cell functioning, independently of the p53 tumour suppressor factor. We observed a higher resistance to caffeine, hydrogen peroxide and to pheromone, but not to high temperature, starvation and osmostress. Measurement of the relative expression levels of target genes of the signalling pathways, involved in the latter stressful stimuli, led us to conclude that such pathways are differently regulated in the presence of E6.

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