A novel murine segmental femoral graft model
Article first published online: 1 JAN 2006
Copyright © 2004 Orthopaedic Research Society
Journal of Orthopaedic Research
Volume 22, Issue 6, pages 1254–1260, November 2004
How to Cite
Tiyapatanaputi, P., Rubery, P. T., Carmouche, J., Schwarz, E. M., O'Keefe, R. J. and Zhang, X. (2004), A novel murine segmental femoral graft model. J. Orthop. Res., 22: 1254–1260. doi: 10.1016/j.orthres.2004.03.017
- Issue published online: 1 JAN 2006
- Article first published online: 1 JAN 2006
- Manuscript Accepted: 11 MAR 2004
- Manuscript Received: 3 MAR 2004
- unrestricted research fellowship from DePuy Inc./a Johnson and Johnson company. E.S.
- Musculoskeletal Transplant Foundation and The National Institute of Health. Grant Number: (AR45971)
- National Institute of Health. Grant Number: (AR46545)
- Orthopedic Research Education Foundation and Musculoskeletal Transplant Foundation
To further understand the cellular and molecular mechanisms underlying cortical bone graft healing, we have developed a novel mouse femur model that permits quantitative and molecular analysis of structural bone graft healing. A 4 mm mid-diaphyseal femoral segment was removed and replaced by either immediate implantation of a fresh autograft, a frozen, genetically identical isograft or a frozen allograft from a different strain of mouse, which was secured with a 22-gauge metal intramedullary pin. Healing was evaluated by radiology, histomorphometry, and in situ hybridization. Autograft repair occurred by endochondral bone formation at the host–graft junction and by intramembranous bone formation along the length of the graft bed at 2 weeks, with maturation and remodeling apparent by 4 weeks. Bone repair in allografts and isografts completely relied on endochondral bone formation at the host–graft cortical junction, with absence of periosteal bone formation along the length of the graft, suggesting that live periosteal cells from the donor tissue are necessary for this response. This small animal model of structural bone grafting can be used to evaluate tissue-engineered allografts and novel bone graft substitutes using quantitative and molecularly defined outcome measures. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.