Effect of selected wound antiseptics on adult articular cartilage (bovine sesamoid bone) in the presence of Escherichia coli and Staphylococcus aureus
Article first published online: 1 JAN 2006
Copyright © 2005 Orthopaedic Research Society
Journal of Orthopaedic Research
Volume 23, Issue 1, pages 127–133, January 2005
How to Cite
Müller, G. and Kramer, A. (2005), Effect of selected wound antiseptics on adult articular cartilage (bovine sesamoid bone) in the presence of Escherichia coli and Staphylococcus aureus. J. Orthop. Res., 23: 127–133. doi: 10.1016/j.orthres.2004.06.003
- Issue published online: 1 JAN 2006
- Article first published online: 1 JAN 2006
- Manuscript Accepted: 9 JUN 2004
- Manuscript Received: 17 FEB 2004
- Deutsche Forschungsgemeinschaft. Grant Number: Mu 929/4-1
- E. coli;
- S. aureus;
- Articular cartilage;
- Wound antiseptic
After removing an inoculum of 108 –109 cfu of Escherichia coli (E. coli) or Staphylococcus aureus (S. aureus) effectively within 2 h from bovine sesamoid bones (bsb) using the iodophore Betaisodona® [0.5 and 1.0% (v/v) PVP-I], the biguanide polihexanide (PHMB) [0.005 and 0.01% (v/v)], and the bispyridinamine Octenidine (Oct) [0.005 and 0.01% (v/v)] the metabolic effect on proteoglycans (PG) in bsb was investigated by subsequent culture of bsb for 7 d and biosynthetically labeling with 35 S-sulfate for a period of 24 h.
The treatment with the iodophore stimulates the incorporation of 35 S-sulfate into PG, whereas that of Oct was toxic. The PHMB treatment was both tolerated and effective only when it was used at low concentrations (0.005%). The antiseptic treatment did not result in an increase of catabolism of PG.
This in vitro study clearly demonstrates that irrigation of cartilage with an antiseptic should be limited to an effective concentration and treatment time compatible with antiseptic function. Iodophores have no negative feedback on cartilage metabolism, moreover, they stimulate chondrocytes in vitro. Cationic antiseptics are not suited as irrigating solutions. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.