Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells

Authors

  • C. W. Chen,

    1. Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
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    • C.W. Chen and Y.H. Tsai contributed equally to this paper

  • Y. H. Tsai,

    1. Institute of Biomedical Materials, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
    2. Institute of Cellular and Molecular Biology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
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    • C.W. Chen and Y.H. Tsai contributed equally to this paper

  • W. P. Deng,

    1. Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
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  • S. N. Shih,

    1. Orthopedic Department, Chang-Gung Memorial hospital, Taoyuan 333, Taiwan
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  • C. L. Fang,

    1. Department of Pathology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
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  • J. G. Burch,

    1. Department of Orthodontics, School of Dental Medicine, Nova Southeastern University, Ft. Lauderdale, FL 33328, USA
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  • W. H. Chen,

    1. Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
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  • W. F. Lai

    Corresponding author
    1. Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
    2. Institute of Biomedical Materials, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
    3. Institute of Cellular and Molecular Biology, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan
    • Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan Tel.: +886 2 87800225/27361661x5210; fax: +886 2 27357714
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Abstract

Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor-beta 1 (TGF-β1) and dexamethasone. Extracellular matrix (ECM) also regulates the differentiation by MPCs. To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. That model exposed to dexamethasone, recombinant human TGF-β1(rhTGF-β1) and collagens. The results showed that MPCs incorporated with dexamethasone and rhTGF-β1 increased proliferation and expression of glycosaminoglycan (GAG) after 14 days. Type II collagen enhanced the GAG synthesis, but did not increase alkaline phosphatase (ALP) activity. When adding dexamethasone and rhTGF-β1 MPCs increased mRNA expression of Sox9. Incorporation with type II collagen, dexamethasone and rhTGF-β1, MPCs induced mRNA expression of aggrecan and enhanced levels of type II collagen, and Sox9 mRNA. In contrast, incorporation with type I collagen, dexamethasone and rhTGF-β1 MPCs reduced levels of aggrecan, and Sox9 mRNA, and showed no type II collagen mRNA. Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.

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