Get access

Using the Microcyte Flow Cytometer To Monitor Cell Number, Viability, and Apoptosis in Mammalian Cell Culture

Authors

  • Claire L. Harding,

    1. Aber Instruments Ltd., Science Park, Aberystwyth, SY23 3AH, U.K.
    Current affiliation:
    1. New Product Development Unit, Bio Products Laboratory, Dagger Lane, Elstree, Hertfordshire, WD6 3BX, U.K.
    Search for more papers by this author
  • David R. Lloyd,

    1. Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.
    Search for more papers by this author
  • Caroline M. McFarlane,

    1. Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.
    Search for more papers by this author
  • Mohamed Al-Rubeai

    Corresponding author
    1. Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.
    • Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.
    Search for more papers by this author

Abstract

The Microcyte is a novel, portable flow cytometer based on diode laser technology whose use has been established for yeast and bacterial analysis. We present data that demonstrate its suitability for routine mammalian cell counting and viability determination. To extend its range of applications in the field of animal cell culture biotechnology, a test to determine the number of apoptotic cells present has been developed for use with the instrument. Apoptosis was induced in hybridoma cell cultures by treatment with camptothecin. Apoptotic cells were labeled with biotinylated Annexin V and then visualized using a streptavidin-allophycocyanin conjugate. Their numbers were counted, and the cell size of the apoptotic cell population was determined using the Microcyte.

Ancillary